Male vs. Female DNA Testing – What are the Differences?

Male vs. Female DNA Testing – What are the Differences?

Male vs. Female DNA Testing – What are the Differences?

You may have heard that there are different DNA tests you can take – both for ancestry testing and confirming family relationships – depending on whether you’re male or female. You may wonder whether the information you can find out about your family is limited by whether you were born male or female. This can sometimes be the case – though there are ways around it.

Some ancestry tests use male Y-DNA to trace paternal ancestry, and mitochondrial DNA (mtDNA) to trace maternal ancestry. While men and women both inherit mtDNA from their mother, only those born male inherit Y-DNA from their father. This means that while men can easily trace their maternal and paternal ancestry using their own DNA, those born female will need a male paternal relative to take a male haplogroup test on their behalf if they wish to learn about their paternal ancestry.

For many DNA tests the sex of the person being tested is irrelevant. This is because of the 23 pairs of chromosomes that make up our DNA, only one pair determines sex. The chromosomes that determine sex are X and Y. Those born genetically male have one X chromosome inherited from their mother, and one Y chromosome inherited from their father. Those bon female have two X chromosomes: one inherited from their mother, and one from their father.

Our remaining 22 chromosomes have nothing to do with determining sex, and are randomly inherited from both our mother and father. These contain the information that determine things like eye color, skin color, hair texture, and so on.

Autosomal DNA Testing

The majority of ancestry and health tests analyze only the autosomal DNA: the DNA contained in 22 of our 23 pairs of chromosomes, which men and women both have. These tests look for genetic markers at places in our genome where variations are known to occur. The genetic variants they look for are known as “SNPs”, or Single Nucleotide Polymorphisms. A SNP is a change of one allele or two alleles: the “letters” that make up our genome. These variations can tell us about our predispositions to certain traits and health conditions, and also serve as ancestry markers.

Ancestry tests such as AncestryDNA and MyHeritage look for variations in our autosomal DNA that can be used to trace the similarities in our unique genetics to global populations. These tests can show your ancestry up to around eight generations back. Unless you connect with a parent on an ancestry website such as 23andMe or AncestryDNA, it can be hard to tell which side of your family your ancestry is on using only autosomal DNA testing.

Some ancestry tests, such as 23andMe and Living DNA, look at both our autosomal DNA, and our mitochondrial DNA inherited from our mother, and the Y-DNA men inherit from their father, in order to trace our maternal and paternal ancestry as well as our autosomal (or “familial”) ancestry.

Tracing Maternal Ancestry

Men and women both inherit mitochondrial DNA (mtDNA) from their mother. Mitochondrial DNA doesn’t mutate often, and so it can be used to trace your maternal ancestry, tracking the migrations of your motherline way back in history.

Companies offering maternal ancestry testing include:

These companies will tell you which maternal haplogroup you belong to, as well as the larger haplogroups your subgroup split off from. Generally, you will be given a map showing the migrations of your maternal ancestors out of or within Africa, and some information about where members of your haplogroup commonly reside today.

The 23andMe maternal haplogroup map

The 23andMe maternal haplogroup map

Tracing Paternal Ancestry

Men, or those born biologically male, can easily trace their paternal ancestry through the Y-DNA contained in the Y chromosome inherited from their father. Like mitochondrial DNA, Y-DNA doesn’t mutate often, and so the rare mutations can be used to trace your paternal ancestry and haplogroup.

Companies offering paternal ancestry testing include:

You can get the same sort of information about your paternal haplogroup as for your maternal one: which paternal haplogroup you belong to, and which of the larger haplogroups your subgroup split off from. You will generally be given a map showing the migrations of your paternal ancestors out of or within Africa, and some information about where members of your haplogroup commonly reside today.

The 23andMe paternal haplogroup map

The 23andMe paternal haplogroup map

Women who want to trace their paternal ancestry will require a male relative on their father’s side to take the test for them.

Eligible relatives include:

If you have no known male relatives on your father’s side, or are unsure which of your relatives are eligible to take a paternal ancestry test for you, then we have a guide for women who wish to trace their paternal ancestry here.

Confirming relationships with Y-DNA and mtDNA

It’s a bit old school, but DNA testing companies sometimes use mtDNA and Y-DNA to confirm familial relationships, instead of genetic markers found in autosomal DNA (which are most commonly used for relationship testing).

Mitochondrial DNA might be used to confirm relationships between siblings, or mothers and their children, or maternal aunts, uncles, and their nephews and nieces. Similarly, Y-DNA can be used to confirm relationships between fathers and sons, brothers, paternal uncles and nephews, and paternal grandfathers and grandsons.

Since not all close relations will share the same mtDNA or Y-DNA, these tests are quite limited, and not applicable to every situation, which is why testing of STRs (Short Tandem Repeats) is more commonly used for testing familial relationships.

Prenatal Sex Testing

There are many companies now offering prenatal sex testing. These tests analyze a sample of the mother’s blood, looking for cell-less DNA from the fetus. They don’t tell you anything about the fetus’ DNA other than the genetic sex, since what they are looking for is the presence of a Y chromosome in the mother’s blood. If a Y chromosome is found, it is supposed the fetus is male. If no Y chromosome is found, it is supposed the fetus is male.

Of course, quite a large sample of the mother’s blood must be tested in order to yield accurate results, and a professional will be needed to draw the sample. (Please bear in mind that any prenatal sex or paternity test that only requires a finger prick and does not require blood to be drawn by a professional will not yield an accurate result.)

Though many companies advertise that tests can be performed after ten weeks, there may not be enough fetal DNA in the mother’s bloodstream to be detected, which could potentially lead to a false result. A male baby may have a false female result if no Y-DNA is detected in the mother’s blood, while cross-contamination with male DNA could lead to a false male result for female babies.

If you choose to get a baby sex test, it is best to do so only in the spirit of curiosity, and not rely too heavily on the results. Additionally, this test should not replace an ultrasound test, or be considered more accurate.


November 17, 2018

My question is with all these matching DNA cousins I would like to know how we can find out if they are related on the Male or Female side of the family for a easier search.

Hi Terrie,

Thank you for commenting.

If you have connected with either your mother or father on 23andMe, then you can filter your DNA match results by which side of your family they are on. I think this is currently the only ancestry site to offer this feature. However, if you're using a site like Ancestry which also has a family tree feature, you can use your relatives' family trees (if they are sharing them) to see how you are related.

I hope this is helpful!

Kind regards,
Harriet Seldon | Services Team

Teresa Hutchinson

October 9, 2019

I’d really like my paternal info. I have a nephew that is my brother’s son. Would testing him provide the info that Im looking for?

Rebecca E Thompson

November 8, 2019

I am trying to find the grandfather of my husband. There are no other children born to his mother and this man. Will any DNA test show information about this man?


January 22, 2020

I’ve recently received the results of a DNA test that I thought was autosomal; however, the results were identical to a Mitochondrial test I had some years ago. No evidence of my Father’s DNA. Is that possible?
Thanks for your help in clearing this up for me.

Jacqueline Day

June 8, 2021

I would like to know, by me getting tested, in which i did , and my sister’s all with the same Mother, are there DNA the same as mine?

Jake Brown

April 24, 2022

April 24th, 2022

Hello there, I obviously my DNA genetics are a XY chromosomes. I have short brown hair, deep blue eyes, aggressive and not to be mess with (only when necessary), and extremely patient.
(23 years old)

P.S. I would sacrifice my life to save my sister’s, as a older brother.

My’s sister’s DNA genetics are a XX chromosomes. she has blonde hair (medium length), deep blue eyes, two side ponytails (medium length), black eye lashes, and a flower clip accessory. (16 years old)

Deborah Simmons

May 19, 2022

How did the Maury show determine paternity in female babies? If the Y only determines paternity in males, how is paternity
definitively determined in females? I already know who my mother is, I need to determine paternity and am female.


August 1, 2022

Y DNA and mtDNA and their direct role in our DNA formation is understood but what role does father’s mother, paternal grandfather’s mother and so on (or in other words mtDNAs of father, his father and so on) play in one’s DNA?
In others words, can DNA testing reveal ancestors from mtDNAs on paternal side?

Wallace W Dickerson

August 16, 2022

Why is not mentioned in this article?

Willerton Brian

October 5, 2022

I have taken the ‘Ancestry’ test, but they told me they couldn’t trace my mother’s father without a sample from him, this is at the crux of the issue with me, how can I trace his lineage back without it?


December 25, 2022

Will the DNA show both sides of mothers and fathers parents. Specially the males side


May 5, 2023

Under the title “Prenatal Sex Testing”, first paragraph, please correct the error found in this sentence, “If no Y chromosome is found, it is supposed the fetus is male.” It should say “female”, not “male”.

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